1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA, pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the following:0.40 g Agarose4.00 mL 50X TBE (4X TAE final)46.00 mL Water1.00 ml 10 mg/mL Ethidium Bromide3. Melt agarose in the microwave4. Seal horizontal gel apparatus. Pour molten agarose onto gel plate to a depth of 4 - 8 mm.5. Insert a comb until its base is 1 mm from the base of the gel. Allow to cool6. Remove comb and submerge in 4X TAE buffer.7. Prepare 6X Loading Dye as:3 mL 100% Glycerol3 mL 0.5 M EDTA, pH 8.03 mg Bromophenol Blue3 mg Xylene Cyanol4 mL Sterile Water10 mL Total Volume8. Add 1/5 volume 6X Loading Dye to sample. Mix well and pellet in the microfuge. 9. Add sample to well with a yellow tip. Do not overflow well. For large samples, either reduce sample in volume prior to adding dye, or load into multiple wells.10. Electrophorese until Bromophenol Blue is near the end of the gel. This dye runs at ~800 b.11. Remove gel and visualize bands under uv light.